Apolipoprotein H/ApoH Antibody [Unconjugated]

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Apolipoprotein H/ApoH was detected in perfusion fixed frozen sections of adult mouse pancreas using Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Apolipoprotein H/ApoH Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Apolipoprotein H/ApoH
  Gly20-Cys345
  Accession # P02749
• Specificity: Detects human Apolipoprotein H/ApoH in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOH
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein H/ApoH Antibody [Unconjugated]

• Activated protein C-binding protein
• Anticardiolipin cofactor
• APC inhibitor
• APOH
• apo-H
• apolipoprotein H (beta-2-glycoprotein I)
• Apolipoprotein H
• B2G1
• B2GP1
• B2GPI
• Beta
• beta(2)GPI
• beta-2-glycoprotein 1
• Beta-2-glycoprotein I
• BG

Background

ApoH (Apolipoprotein H; also beta 2-Glycoprotein 1/ beta 2-GPI) is a 50 kDa, secreted, variably glycosylated, monomeric member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway, and binds to exposed phospholipids on apoptotic cells. Mature human ApoH is 326 amino acids (aa) in length (aa 20‑345). It contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two ApoH isoforms exist, one showing a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOH
• Uniprot:
  • P02749(Human)