Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free

Western Blot: Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free [NBP2-80580] - Detection of Apo A5 in HepG2 cell lysate. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free [NBP2-80580] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using 5 ug/ml conc. of Apolipoprotein A5 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium c

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free [NBP2-80580] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Western Blot: Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free [NBP2-80580] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard. Image from the standard format of this antibody.

Western Blot: Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free [NBP2-80580] - Analysis of ApoA5 in HepG2 lysates Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC/IF (-)
• Clone: 1G5G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.9 mg/ml

Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free Summary

• Immunogen: Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
• Localization: Secreted
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: This Apolipoprotein A5 antibody is useful in ELISA, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1.9 mg/ml
• Purity: Protein G purified

Alternate Names for Apolipoprotein A5 Antibody (1G5G9) - Azide and BSA Free

• APOAV
• apo-AV
• APOA-V
• Apolipoprotein A5
• apolipoprotein A-V
• FLJ97995
• MGC126836
• RAP3MGC126838
• Regeneration-associated protein 3

Background

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOA5