Apolipoprotein A5 Antibody (1G5G9) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
1G5G9
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Apolipoprotein A5 Antibody (1G5G9) [DyLight 405] Summary

Immunogen
Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOA5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This Apolipoprotein A5 antibody is useful in ELISA, Immunohistochemistry paraffin embedded sections and Western blot.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Apolipoprotein A5 Antibody (1G5G9) [DyLight 405]

  • APOAV
  • apo-AV
  • APOA-V
  • Apolipoprotein A5
  • apolipoprotein A-V
  • FLJ97995
  • MGC126836
  • RAP3MGC126838
  • Regeneration-associated protein 3

Background

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB110-55454B
DyLight 488 Labeled NB110-55454G
DyLight 550 Labeled NB110-55454R
DyLight 650 Labeled NB110-55454C
FITC Labeled NB110-55454F
HRP Labeled NB110-55454H

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Bioinformatics

Gene Symbol APOA5