Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

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Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
4.69 - 300 ng/mL
Sensitivity
2.81 ng/mL

Order Details

Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.
Standard Curve Range
4.69 - 300 ng/mL
Sensitivity
2.81 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.5%
Intra-Assay
CV% < 5.09%
Spike Recovery
88-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOA4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Apolipoprotein A-IV/ApoA4 ELISA Kit (NBP2-68246). (Showing 1 - 1 of 1 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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Bioinformatics

Gene Symbol APOA4