Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

ELISA: Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68246] - Samples were spiked with high concentrations of Human Apolipoprotein A-IV/ApoA4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68246] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 4.69 - 300 ng/mL
• Sensitivity: 2.81 ng/mL

Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.
• Standard Curve Range: 4.69 - 300 ng/mL
• Sensitivity: 2.81 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.5%
• Intra-Assay: CV% < 5.09%
• Spike Recovery: 88-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APOA4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

• apo-AIV
• ApoA-IV
• Apolipoprotein A4
• Apolipoprotein AIV
• Apolipoprotein A-IV
• MGC142154
• MGC142156

Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Apolipoprotein A-IV/ApoA4 ELISA Kit (NBP2-68246)(2)

Publications using NBP2-68246

• Matsuo S, Moriyama Y, Ushida T et al. Increased levels of APOA4 in cord blood in maternal major depressive disorder Research Square 2023-09-22 (ELISA)
• Matsuo S, Moriyama Y, Ushida T et al. Elevated levels of apolipoprotein A4 in umbilical cord serum from the maternal major depressive disorder. The journal of obstetrics and gynaecology research 2024-09-25 [PMID: 39319776]

FAQs for Apolipoprotein A-IV/ApoA4 ELISA Kit (NBP2-68246). (Showing 1 - 2 of 2 FAQs).

1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
   • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APOA4