![Immunocytochemistry/Immunofluorescence: APOBEC3B Antibody [NBP2-56411] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/APOBEC3B Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56411-img0001.jpg "Immunocytochemistry/Immunofluorescence: APOBEC3B Antibody [NBP2-56411] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ELRFLDLVPSLQLDPAQIYRVTWFI |
| Specificity | Specificity of human APOBEC3B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | APOBEC3B |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in IHC-P reported in secitific publication PMID: 32560902 |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
| Publication using NBP2-56411 | Applications | Species |
|---|---|---|
| Kono T, Hoover P, Poropatich K et al. Activation of DNA damage repair factors in HPV positive oropharyngeal cancers Virology Jan 1 2020 [PMID: 32560902] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Diseases for APOBEC3B Antibody (NBP2-56411)Discover more about diseases related to APOBEC3B Antibody (NBP2-56411).
| Pathways for APOBEC3B Antibody (NBP2-56411)View related products by pathway.
|
PTMs for APOBEC3B Antibody (NBP2-56411)Learn more about PTMs related to APOBEC3B Antibody (NBP2-56411).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | APOBEC3B |
![Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-88592] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/images2/APOBEC3G-Antibody-Immunohistochemistry-Paraffin-NBP1-88592-img0009.jpg)
![Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-88592] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/APOBEC3G-Antibody-Immunohistochemistry-Paraffin-NBP1-88592-img0003.jpg)
![Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-88592] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/images2/APOBEC3G-Antibody-Immunohistochemistry-Paraffin-NBP1-88592-img0005.jpg)
![Western Blot: Citidine Deaminase Antibody [NBP2-39019] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/images2/Citidine-Deaminase-Antibody-Western-Blot-NBP2-39019-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Citidine Deaminase Antibody [NBP2-39019] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/Citidine-Deaminase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39019-img0004.jpg)
![Immunohistochemistry-Paraffin: Citidine Deaminase Antibody [NBP2-39019] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/Citidine-Deaminase-Antibody-Immunohistochemistry-Paraffin-NBP2-39019-img0005.jpg)
![Western Blot: APOBEC1 Antibody [NBP1-06977] - (1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/APOBEC1-Antibody-Western-Blot-NBP1-06977-img0003.jpg)
![Western Blot: APOBEC3F Antibody [NBP2-92770] - Analysis of extracts of various cell lines, using APOBEC3F at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/images2/APOBEC3F Antibody-Western Blot-NBP2-92770-img0004.jpg)
![Immunohistochemistry-Paraffin: APOBEC3F Antibody [NBP2-92770] - Paraffin-embedded rat ovary using APOBEC3F.](http://images.novusbio.com/images2/APOBEC3F-Antibody-Immunohistochemistry-Paraffin-NBP2-92770-img0003.jpg)
![Immunohistochemistry-Paraffin: APOBEC3F Antibody [NBP2-92770] - Paraffin-embedded human lung cancer using APOBEC3F.](http://images.novusbio.com/images2/APOBEC3F-Antibody-Immunohistochemistry-Paraffin-NBP2-92770-img0001.jpg)
![Western Blot: DCK Antibody (10412) [NBP2-43639] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (10412) diluted at 1:500.](http://images.novusbio.com/images2/DCK-Antibody-(10412)-Western-Blot-NBP2-43639-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DCK Antibody (10412) [NBP2-43639] - DCK antibody (10412) detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/DCK-Antibody-(10412)-Immunocytochemistry-Immunofluorescence-NBP2-43639-img0007.jpg)
![Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Analysis of human lung cancer. DCK antibody [10412] diluted at 1:1000.](http://images.novusbio.com/images2/DCK-Antibody-(10412)-Immunohistochemistry-Paraffin-NBP2-43639-img0002.jpg)
![Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.](http://images.novusbio.com/images2/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB200-527-img0001.jpg)
![IL-4 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-4 [Unconjugated]")
![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg)
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/images2/uracil-DNA glycosylase Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)
![Western Blot: APOBEC3C Antibody [NBP1-00131] - (0.5ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/APOBEC3C Antibody-Western Blot-NBP1-00131-img0002.jpg)
![Western Blot: SCARA3 Antibody [NBP1-32130] - Sample(30 ug of whole cell lysate)A:Hep G2. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/images2/SCARA3 Antibody-Western Blot-NBP1-32130-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SCARA3 Antibody [NBP1-32130] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: SCARA3 protein stained by SCARA3 antibody, Internal diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/images2/SCARA3 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32130-img0002.jpg)
![Immunohistochemistry-Paraffin: SCARA3 Antibody [NBP1-32130] - CL1-0 xenograft, using SCARA3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/SCARA3 Antibody-Immunohistochemistry-Paraffin-NBP1-32130-img0003.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum, small intestine, stomach and testis using Anti-Adenosine Deaminase/ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0027.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0028.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-87404-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.](http://images.novusbio.com/images2/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-335-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0005.jpg)
![Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/images2/ACF-Antibody-Western-Blot-NBP1-90271-img0005.jpg)
![Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human colon shows moderate nuclear/cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/ACF-Antibody-Immunohistochemistry-Paraffin-NBP1-90271-img0010.jpg)
![Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/images2/ACF-Antibody-Immunohistochemistry-Paraffin-NBP1-90271-img0001.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/images2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Western Blot: APOBEC3A Antibody [NBP1-88999] - Analysis in control (vector only transfected HEK293T lysate) and APOBEC3A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/APOBEC3A-Antibody-Western-Blot-NBP1-88999-img0014.jpg)
![Immunohistochemistry-Paraffin: APOBEC3A Antibody [NBP1-88999] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/images2/APOBEC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-88999-img0012.jpg)
![Immunohistochemistry-Paraffin: APOBEC3A Antibody [NBP1-88999] - Staining of human lung shows moderate cytoplasmic positivity in lymphoid cells and a subset of pneumocytes.](http://images.novusbio.com/images2/APOBEC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-88999-img0009.jpg)
![Western Blot: Deoxycytidylate deaminase Antibody [NBP1-87424] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-160](http://images.novusbio.com/images2/Deoxycytidylate-deaminase-Antibody-Western-Blot-NBP1-87424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Deoxycytidylate deaminase Antibody [NBP1-87424] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/images2/Deoxycytidylate deaminase Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87424-img0008.jpg)
![Immunohistochemistry-Paraffin: Deoxycytidylate deaminase Antibody [NBP1-87424] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/images2/Deoxycytidylate-deaminase-Antibody-Immunohistochemistry-Paraffin-NBP1-87424-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")