APEH Antibody - BSA Free

Immunoprecipitation: APEH Antibody [NBP3-38208] - Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug APEH antibody. Western blot was performed from the immunoprecipitate using APEH antibody at a dilution of 1:1000.

Western Blot: APEH Antibody [NBP3-38208] - Western blot analysis of various lysates, using [KO Validated] APEH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Western Blot: APEH Antibody [NBP3-38208] - Western Blot analysis of lysates from wild type(WT) and APEH knockout (KO) 293T(KO) cells, using [KO Validated] APEH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APEH Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit APEH Antibody - BSA Free (NBP3-38208) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
  
  Sequence:
  MERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APEH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for APEH Antibody - BSA Free

• AARE
• ACPH
• acylamino-acid-releasing enzyme
• acylaminoacyl-peptidase
• Acyl-peptide hydrolase
• APH
• D3F15S2
• D3S48EEC 3.4.19.1
• DNF15S2
• MGC2178
• N-acylaminoacyl-peptide hydrolase
• OPH
• Oxidized protein hydrolase

Background

The APEH gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated aminoacid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is furtherprocessed to acetate and a free

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APEH