AP-2 beta/TFAP2B Antibody

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody diluted at 1:500.

Immunoprecipitation: AP-2 beta/TFAP2B Antibody [NBP2-20606] - IP samples: MCF-7 whole cell extract A. 30 ug MCF-7 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of AP2 beta protein by 4 ug AP2 beta antibody 7.5 % SDS-PAGE The mmunoprecipitated AP2 beta protein was detected by AP2 beta antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca, Ch, Xp, Ze
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

AP-2 beta/TFAP2B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AP-2 beta/TFAP2B. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (100%), Porcine (99%), Bovine (100%), Zebrafish (94%), Xenopus (99%), Chicken (99%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFAP2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AP-2 beta/TFAP2B Antibody

• activating enhancer binding protein 2 beta
• Activating enhancer-binding protein 2-beta
• AP2 beta
• AP-2 beta
• AP-2B
• AP2-B
• AP2-beta
• CHAR
• MGC21381
• TFAP2B
• transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
• transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)
• transcription factor AP-2-beta

Background

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TFAP2B
• Uniprot:
  • Q92481()