ANP32A Antibody - BSA Free

Western Blot: ANP32A Antibody [NBP2-49385] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-49385] - Staining of human cell line RH-30 shows localization to nucleoplasm, cytosol & centrosome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ANP32A Antibody [NBP2-49385] - Staining of human colon shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ANP32A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ANP32A Antibody - BSA Free (NBP2-49385) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KCPNLTHLNLSGNKIKDLSTIEPLK
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANP32A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ANP32A Recombinant Protein Antigen (NBP2-49385PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ANP32A Antibody - BSA Free

• acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• acidic leucine-rich nuclear phosphoprotein 32 family member A
• Acidic nuclear phosphoprotein pp32
• cerebellar leucine rich acidic nuclear protein
• I1PP2A
• inhibitor-1 of protein phosphatase-2A
• LANPPutative HLA-DR-associated protein I
• Leucine-rich acidic nuclear protein
• MAPMC15orf1
• mapmodulin
• PHAP1MGC150373
• PHAPIMGC119787
• Potent heat-stable protein phosphatase 2A inhibitor I1PP2A
• PP32
• putative human HLA class II associated protein I

Background

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANP32A