Annexin A6 Overexpression Lysate (Native)

Images

 
Western Blot: Annexin A6 Overexpression Lysate (Adult Normal) [NBL1-07566] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Annexin A6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202086 and based on accession number NM_001155. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens annexin A6 (ANXA6), transcript variant 1, mRNA.
Gene
ANXA6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
75.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Annexin A6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Annexin A6 Overexpression Lysate (Native)

  • 67 kDa calelectrin
  • Annexin A6
  • annexin VI (p68)
  • Annexin VI
  • Annexin-6
  • ANX6
  • ANXA6
  • calcium-binding protein p68
  • Calelectrin
  • calphobindin II
  • calphobindin-II
  • CBP68
  • chromobindin-20
  • CPB-II
  • CPB-II
  • Lipocortin VI
  • p68
  • p70
  • Protein III

Background

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is alternatively spliced, giving rise to two isoforms that differ by a 6-amino acid insertion at the start of the seventh repeat. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Annexin A6 Lysate (NBL1-07566) (0)

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Bioinformatics

Gene Symbol ANXA6
Entrez