Human Annexin A6 ELISA Kit (Colorimetric)

ELISA: Human Annexin A6 ELISA Kit (Colorimetric) [NBP3-18211] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and cell culture supernates
• Standard Curve Range: 0.156-10 ng/ml
• Sensitivity: 0.069 ng/ml

Human Annexin A6 ELISA Kit (Colorimetric) Summary

• Description: The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Annexin A6 in the sample, this Human Annexin A6 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Annexin A6 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Standard Curve Range: 0.156-10 ng/ml
• Sensitivity: 0.069 ng/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 10
• Spike Recovery: 83-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ANXA6

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Annexin A6 ELISA Kit (Colorimetric)

• 67 kDa calelectrin
• Annexin A6
• annexin VI (p68)
• Annexin VI
• Annexin-6
• ANX6
• ANXA6
• calcium-binding protein p68
• Calelectrin
• calphobindin II
• calphobindin-II
• CBP68
• chromobindin-20
• CPB-II
• CPB-II
• Lipocortin VI
• p68
• p70
• Protein III

Background

The Annexins constitute a family of structurally-related, relatively abundant proteins that exhibit Ca2+-dependent binding to phospholipids. Annexins function in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, inhibition of phospholipase A2, inhibition of coagulation and mediation of cell-matrix interactions. Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function. Two alternative splicing isoforms of Annexin A13 exist, both of which bind to rafts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Annexin A6 ELISA Kit (NBP3-18211). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANXA6