Annexin A6 Antibody (686921) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 686921
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Annexin A6 Antibody (686921) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Annexin A6
  Ala2-Asp673
  Accession # P08133
• Specificity: Detects human Annexin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A8, A9, A10, A11, or A13 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A6 Antibody (686921) [Alexa Fluor® 647]

• 67 kDa calelectrin
• Annexin A6
• annexin VI (p68)
• Annexin VI
• Annexin-6
• ANX6
• ANXA6
• calcium-binding protein p68
• Calelectrin
• calphobindin II
• calphobindin-II
• CBP68
• Chromobindin-20
• CPB-II
• CPB-II
• Lipocortin VI
• p68
• p70
• Protein III

Background

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60-70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5‑7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.