Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free

Flow Cytometry: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-14C-10F12 (NBP3-20093, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - HepG2(A) (0.001ug/ml), A431(B) (0.001ug/ml), and HeLa(C) (0.01ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-14C-10F12 (NBP3-20093), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-14C-10F12 (NBP3-20093) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20093, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, MS
• Clone: SAIC-14C-10F12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free (NBP3-20093) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide "DEGNYLDDALVR" derived from Annexin A4 conjugated to KLH.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANXA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Mass Spectrometry
  • Western Blot
• Application Notes: This Ab can be used in immunoMRM.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free

• AIV
• Annexin A4
• annexin IV (placental anticoagulant protein II)
• Annexin IV
• annexin-4
• ANX4
• ANX4Carbohydrate-binding protein p33/p41
• ANXA4
• Chromobindin-4
• chromobindin-4,35-beta calcimedin
• DKFZp686H02120
• Endonexin I
• Lipocortin IV
• MGC75105
• P32.5
• PAP-II
• PIG28
• Placental anticoagulant protein II
• PP4-X
• proliferation-inducing gene 28
• proliferation-inducing protein 28
• Protein II
• ZAP36

Background

Annexin A4, also known as Annexin A4, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin A4 is almost exclusively expressed in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA4