![Western Blot:[NBP3-30214]-Detection of human ANKLE2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-ANKLE2 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. ANKLE2 was also immunoprecipitated by rabbit anti-ANKLE2 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30214_rabbit-ankle2-pab-231202511585568.jpg "Western Blot:[NBP3-30214]-Detection of human ANKLE2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-ANKLE2 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. ANKLE2 was also immunoprecipitated by rabbit anti-ANKLE2 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit ANKLE2 Antibody (NBP3-30214) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | between 700 and 750 |
| Assay Type | [Assay Type] |
| Sample Volume | [Sample Volume] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANKLE2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-buffered Saline 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ANKLE2 Antibody (NBP3-30214)Find related products by research area.
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| Gene Symbol | ANKLE2 |
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")