![Immunohistochemistry: LEM3 Antibody - BSA Free [NBP3-48163] - Immunohistochemistry of paraffin-embedded rat brain tissue slide using (LEM3 Antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-48163_rabbit-lem3-pab-bsa-free-28420251138082.jpg "Immunohistochemistry: LEM3 Antibody - BSA Free [NBP3-48163] - Immunohistochemistry of paraffin-embedded rat brain tissue slide using (LEM3 Antibody) at dilution of 1:100")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit LEM3 Antibody - BSA Free (NBP3-48163) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | ankyrin repeat and LEM domain containing 1. (Uniprot# Q8NAG6) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANKLE1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol, pH (7.3) |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for LEM3 Antibody (NBP3-48163)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANKLE1 |
![Western Blot: LEM3 Antibody - BSA Free [NBP3-48163] - Hela cells were subjected to SDS PAGE followed by western blot with (LEM3 Antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-48163_rabbit-lem3-pab-bsa-free-284202511433275.jpg "Western Blot: LEM3 Antibody - BSA Free [NBP3-48163] - Hela cells were subjected to SDS PAGE followed by western blot with (LEM3 Antibody) at dilution of 1:500")
![Immunocytochemistry/ Immunofluorescence: LEM3 Antibody - BSA Free [NBP3-48163] - Immunofluorescent analysis of Hela cells using ( (LEM3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)](http://images.novusbio.com/fullsize/nbp3-48163_rabbit-lem3-pab-bsa-free-28420251134732.jpg "Immunocytochemistry/ Immunofluorescence: LEM3 Antibody - BSA Free [NBP3-48163] - Immunofluorescent analysis of Hela cells using ( (LEM3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)")
![Bioactivity Flt-3 Ligand/FLT3L [Unconjugated]](https://images.novusbio.com/images/308-fk_recombinant-human-flt-3-ligand-flt3l-protein-bioactivity-2132023173432.jpg)
![Immunohistochemistry Neogenin Antibody [Unconjugated]](https://images.novusbio.com/images2/Neogenin_AF1079_Immunohistochemistry_17347.jpg)
![Western Blot Neogenin Antibody [Unconjugated]](https://images.novusbio.com/images2/Neogenin_AF1079_Western_Blot_19964.jpg)
![Simple Western Neogenin Antibody [Unconjugated]](https://images.novusbio.com/images2/Neogenin_AF1079_Simple_Western_20102.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")