Mohawk homeobox Antibody (OTI6B12)

Images

 
Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mohawk homeobox.
Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Characterization of TLC cultured in chondrogenic pellets. Representative images of histological and immunohistochemical staining of TLC pellets (CH ...read more
Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI6B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Mohawk homeobox Antibody (OTI6B12) Summary

Immunogen
Full length human recombinant protein of human MKX(NP_775847) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MKX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
39.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-45863 in the following applications:

Publications
Read Publication using NBP2-45863.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mohawk homeobox Antibody (OTI6B12)

  • C10orf48
  • homeobox protein Mohawk
  • IFRX
  • Iroquois family related homeodomain protein
  • iroquois homeobox protein-like 1
  • IRXL1chromosome 10 open reading frame 48
  • MGC39616
  • mohawk homeobox

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mohawk homeobox Antibody (NBP2-45863)(1)

We have publications tested in 1 application: IHC.


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IHC
(1)
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Review for Mohawk homeobox Antibody (NBP2-45863) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-45863:
Filter by Applications
ELISA
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
ELISA Mohawk homeobox NBP2-45863
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4
reviewed by:
Verified Customer
ELISA Human 01/22/2018
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Summary

ApplicationELISA
Sample TestedCell lysate (human dermal fibroblasts - HDFa)
SpeciesHuman
LotW001

Comments

CommentsCapture antibody: anti-MKX antibody NBP2-45863 (2ug/mL) - overnight incubation at 4ºC
Protein: Recombinant human Mohawk homeobox protein from another supplier at different concentrations (62,5-2000ng/ml)
Detection: Rabbit pAb to Mohawk homeobox from another supplier - 1/100 1h at room temperature
Secundary: Goat pAb to Rb IgG (HRP) from another supplier - 1/5000 1h at room temperature
Sample type: Cell lysate (human dermal fibroblasts - HDFa)

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Mohawk homeobox Antibody (NBP2-45863) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Mohawk homeobox Antibody (NBP2-45863)

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Verified Customer
01/22/2018
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol MKX