Mohawk homeobox Antibody (OTI6B12)

Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mohawk homeobox.

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Characterization of TLC cultured in chondrogenic pellets. Representative images of histological and immunohistochemical staining of TLC pellets (CH medium) for COL2, SOX9, COL10, RUNX2, MKX, TNAP, and ENPP1. COL2 positive control was obtained with a chondrosarcoma sample. HE: Hematoxylin and eosin. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2077-0383/8/10/1544), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI6B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Mohawk homeobox Antibody (OTI6B12) Summary

• Immunogen: Full length human recombinant protein of human MKX(NP_775847) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MKX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 39.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Mohawk homeobox Antibody (OTI6B12)

• C10orf48
• homeobox protein Mohawk
• IFRX
• Iroquois family related homeodomain protein
• iroquois homeobox protein-like 1
• IRXL1chromosome 10 open reading frame 48
• MGC39616
• mohawk homeobox

Background

MKX is a member of an Iroquois (IRX) family-related class of 'three-amino acid loop extension' (TALE)atypical homeobox proteins characterized by 3 additional amino acids in the loop region between helix I and helix IIof the homeodomain (Anderson et al., 2006 (PubMed 16408284)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mohawk homeobox Antibody (NBP2-45863)(1)

Publication using NBP2-45863

• Darrieutort-Laffite C, Arnolfo P, Garraud T et al. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner J Clin Med [PMID: 31561454] (IF/IHC)

Review for Mohawk homeobox Antibody (NBP2-45863) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 01/22/2018

Summary

• Application: ELISA
• Sample Tested: Cell lysate (human dermal fibroblasts - HDFa)
• Species: Human
• Lot: W001

Comments

• Comments: Capture antibody: anti-MKX antibody NBP2-45863 (2ug/mL) - overnight incubation at 4ºC
  Protein: Recombinant human Mohawk homeobox protein from another supplier at different concentrations (62,5-2000ng/ml)
  Detection: Rabbit pAb to Mohawk homeobox from another supplier - 1/100 1h at room temperature
  Secundary: Goat pAb to Rb IgG (HRP) from another supplier - 1/5000 1h at room temperature
  Sample type: Cell lysate (human dermal fibroblasts - HDFa)

Bioinformatics

• Gene Symbol: MKX