Protocadherin-15 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293T human embryonic kidney cell line and HEK293T cell line spiked with Recombinant Human Protocadherin-15 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ...read more
Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Sheep Anti-Human Protocadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6729) at 10 µg/mL for ...read more
Protocadherin‑15 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Protocadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6729) at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Protocadherin-15 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin-15
Gln27-Ala1376
Accession # Q96QU1
Specificity
Detects human Protocadherin-15 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PCDH15
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF6729 in the following applications:

Publications
Read Publication using
AF6729 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-15 Antibody [Unconjugated]

  • autosomal recessive 23
  • cadherin-related family member 15
  • CDHR15
  • DFNB23
  • DKFZp667A1711
  • PCDH15
  • Protocadherin15
  • Protocadherin-15
  • protocadherin-related 15
  • USH1F

Background

Protocadherin 15 (PCDH-15) is a 250-270 kDa "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereoclila in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929 amino acids (aa) in length. It contains a 1350aa extracellular domain (ECD) (aa 27-1376) plus a 558 aa cytoplasmic region. There are 11 cadherin domains in the ECD (aa 40-1259). Multiple splice forms are reported. One is a soluble 110 kDa form that shows a five aa substitution for aa 957-1955. Another contains a seven aa substitution for aa 1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa 27-1376 (the ECD), human PCDH-15 shares 95% aa identity with mouse PCDH-15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protocadherin-15 Antibody (AF6729)(1)

We have publications tested in 1 confirmed species: Xenopus.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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(1)
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Reviews for Protocadherin-15 Antibody (AF6729) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF6729:
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WB
(1)
IHC-Fr
(1)
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(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Protocadherin-15 AF6729
Enlarge
5
reviewed by:
Anonymous
WB Human 10/02/2018
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 transfected cells,HEK 293 transfected
SpeciesHuman
Lot1806A

Comments

CommentsPVDF membrane was blocked with 3% milk for 1 hour at room temperature. Primary antibody sheep anti-Pcdh15 was used at a concentration 2ug/mL in 3% milk and the membrane incubated overnight at 4C. Secondary antibody anti-sheep-HRP was used at a dilution 1:2500 in 3% milk and incubated for 1 hour at room temperature.
Immunohistochemistry-Frozen Protocadherin-15 AF6729
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 09/25/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedOrgan of Corti (inner ear)
SpeciesMouse
Lot1806A

Comments

CommentsP1 mouse organ of Corti was paraformaldehyde fixed (4%) for 30 min, blocked 1 hour in PBS Triton X-100 0.1% containing 10% fetal calf serum and then incubated overnight with sheep anti-Pcdh15 (1:200, green) in blocking solution. Secondary antibody was Alexa488 anti-sheep (1:200). Tissue was counterstained with phalloidin (red).

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
10/02/2018
Application: WB
Species: Human

Anonymous
09/25/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PCDH15
Uniprot