Angiopoietin-like Protein 7/ANGPTL7 Antibody

Western blot shows lysates of rat eye tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Angiopoietin-like Protein 7/ANGPTL7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Angiopoietin-like Protein 7/ANGPTL7 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Angiopoietin-like Protein 7/ANGPTL7
  Gln22-Pro337
  Accession # Q8R1Q3
• Specificity: Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ANGPTL7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 7/ANGPTL7 Antibody

• angiopoietin-like 7
• Angiopoietin-like factor
• Angiopoietin-like Protein 7
• angiopoietin-related protein 7
• ANGPTL7
• AngX
• CDT6
• CDT6angiopoietin-like factor (CDT6)
• Cornea-derived transcript 6 protein
• dJ647M16.1
• RP4-647M16.2

Background

Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Angiopoietin-like Protein 7/ANGPTL7 Antibody (AF4960). (Showing 1 - 1 of 1 FAQ).

1. This product says it can only be used with mouse so why does the picture say it was used in rat?
   • AF4960 is manufactured by our sister company, R&D Systems. The antibody was tested against rat lysate by Western blotting, however rat has not been listed as a validated species since a full range of tests with rat samples have not yet been performed.

Bioinformatics

• Gene Symbol: ANGPTL7
• Uniprot:
  • Q8R1Q3()