Serum Amyloid A1/A2 Antibody [Unconjugated]

Images

 
Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal ...read more
Serum Amyloid A1/A2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) at 15 µg/mL overnight at 4 ...read more
Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate ...read more
Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by ...read more
Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Serum Amyloid A1/A2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Serum Amyloid A1
Gly20-Tyr122
Accession # P05366
Specificity
Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) SAA1, rhSAA2, recombinant mouse (rm) SAA3, and rmSAA4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SAA1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2948 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serum Amyloid A1/A2 Antibody [Unconjugated]

  • Serum Amyloid A1/A2

Background

Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serum Amyloid A1/A2 Antibody (AF2948)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Cell Culture, ELISA Development, IHC, Western Blot.


Filter By Application
Cell Culture
(1)
ELISA Development
(2)
IHC
(3)
Western Blot
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF2948 Applications Species
H Zheng, H Li, J Zhang, H Fan, L Jia, W Ma, S Ma, S Wang, H You, Z Yin, X Li Serum amyloid A exhibits pH dependent antibacterial action and contributes to host defense against Staphylococcus aureus cutaneous infection J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31819008] (Western Blot, Mouse) Western Blot Mouse
E Wicker, L Benton, K George, W Furlow, S Villapol Serum Amyloid A Protein as a Potential Biomarker for Severity and Acute Outcome in Traumatic Brain Injury Biomed Res Int, 2019;2019(0):5967816. 2019 [PMID: 31119176] (Western Blot, Mouse) Western Blot Mouse
H Wang, M Blackall, L Sominsky, SJ Spencer, R Vlahos, M Churchill, S Bozinovski Increased hypothalamic microglial activation after viral-induced pneumococcal lung infection is associated with excess serum amyloid A production J Neuroinflammation, 2018;15(1):200. 2018 [PMID: 29980196] (IHC, Mouse) IHC Mouse
BH Hinrichs, JD Matthews, D Siuda, MN O'Leary, AA Wolfarth, BJ Saeedi, A Nusrat, AS Neish Serum Amyloid A1 is an epithelial pro-restitutive factor Am. J. Pathol., 2018;0(0):. 2018 [PMID: 29366677] (Cell Culture, Human) Cell Culture Human
X Li, Y Lyu, J Shen, Y Mu, L Qiang, L Liu, K Araki, BP Imbimbo, KI Yamamura, S Jin, Z Li Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci Lab. Invest., 2018;0(0):. 2018 [PMID: 29330472] (IHC, Mouse) IHC Mouse
Y Zhang, Y Wei, B Jiang, L Chen, H Bai, X Zhu, X Li, H Zhang, Q Yang, J Ma, Y Xu, J Ben, DC Christiani, Q Chen Scavenger receptor A1 prevents metastasis of non-small cell lung cancer via suppression of macrophage serum amyloid A1 Cancer Res, 2017;0(0):. 2017 [PMID: 28202524] (IHC, Human) IHC Human
Malik P, Berisha SZ, Santore J Zymosan-mediated inflammation impairs in vivo reverse cholesterol transport. J. Lipid Res., 2011;52(5):951-7. 2011 [PMID: 21335620] (Western Blot, Mouse) Western Blot Mouse
Tasaki M, Ueda M, Ochiai S, Tanabe Y, Murata S, Misumi Y, Su Y, Sun X, Shinriki S, Jono H, Shono M, Obayashi K, Ando Y Transmission of circulating cell-free AA amyloid oligomers in exosomes vectors via a prion-like mechanism. Biochem. Biophys. Res. Commun., 2010;400(4):559-62. 2010 [PMID: 20807507] (Western Blot, Mouse) Western Blot Mouse
Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice. J. Nutr., 2007;137(7):1776-82. 2007 [PMID: 17585030] (ELISA Development, Mouse) ELISA Development Mouse
Zlobec I, Lugli A, Baker K, Roth S, Minoo P, Hayashi S, Terracciano L, Jass JR Role of APAF-1, E-cadherin and peritumoral lymphocytic infiltration in tumour budding in colorectal cancer. J. Pathol., 2007;212(3):260-8. 2007 [PMID: 17516584] (ELISA Development, Mouse) ELISA Development Mouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF2948

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Bioinformatics

Gene Symbol SAA1
Uniprot