Angiopoietin-like Protein 4/ANGPTL4 Overexpression Lysate (Native)

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Western Blot: ANGPTL4 Overexpression Lysate (Adult Normal) [NBL1-07527] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ANGPTL4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Angiopoietin-like Protein 4/ANGPTL4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205651 and based on accession number NM_139314. \ The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens angiopoietin-like 4 (ANGPTL4), transcript variant 1, mRNA.
Gene
ANGPTL4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Angiopoietin-like Protein 4/ANGPTL4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Angiopoietin-like Protein 4/ANGPTL4 Overexpression Lysate (Native)

  • Angiopoietin like Protein 4
  • angiopoietin-like 4
  • Angiopoietin-like Protein 4
  • ANGPTL4
  • ARP4fasting-induced adipose factor
  • FIAF
  • FIAFhepatic angiopoietin-related protein
  • Hepatic fibrinogen/angiopoietin-related protein
  • HFARP
  • HFARPANGPTL2
  • NL2
  • peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
  • PGAR
  • PGARangiopoietin-related protein 4
  • pp1158
  • PPARG angiopoietin related protein

Background

This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Angiopoietin-like Protein 4/ANGPTL4 Lysate (NBL1-07527) (0)

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Bioinformatics

Gene Symbol ANGPTL4
Entrez