Angiopoietin-like Protein 2/ANGPTL2 Antibody - BSA Free

Western Blot: Angiopoietin-like Protein 2/ANGPTL2 Antibody [NBP1-79557] - Deglycosylation of Angptl2-GST. Purified Angptl2-GST was incubated with in the absence (Lane A) or presence of PNGase F (Lane B) at 37C for 5 h and then, along with commercially available human GST-Angptl2 (Novus Biologicals; Catalog number H00023452-P01; Lane C), resolved on SDS-PAGE (10% acrylamide), transferred to nitrocellulose (0.2 um), and then visualized by immunoblot analysis using an Angptl2-specific antibody. Image collected and cropped by CiteAb from the following publication (//springerplus.springeropen.com/articles/10.1186/2193-1801-3-337) licensed under a CC-BY license.

Western Blot: Angiopoietin-like Protein 2/ANGPTL2 Antibody [NBP1-79557] - 721_B cell lysate, concentration 0.2-1 ug/ml.

Western Blot: Angiopoietin-like Protein 2/ANGPTL2 Antibody [NBP1-79557] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml

Deglycosylation of Angptl2-GST. Purified Angptl2-GST was incubated with in the absence (Lane A) or presence of PNGase F (Lane B) at 37°C for 5 h and then, along with commercially available human GST-Angptl2 (Novus Biologicals; Catalog number H00023452-P01; Lane C), resolved on SDS-PAGE (10% acrylamide), transferred to nitrocellulose (0.2 μm), and then visualized by immunoblot analysis using an Angptl2-specific antibody.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Angiopoietin-like Protein 2/ANGPTL2 Antibody - BSA Free Summary

• Description: The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
• Immunogen: Synthetic peptide directed towards the N terminal of human ANGPTL2The immunogen for this antibody is ANGPTL2. Peptide sequence NSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVK. The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPTL2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Angiopoietin-like Protein 2/ANGPTL2 Antibody - BSA Free

• angiopoietin-like 2
• Angiopoietin-like Protein 2
• angiopoietin-related protein 2
• ANGPTL2
• ANGRP2
• ARP2HARP
• MGC8889

Background

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largelyspecific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation ofblood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a functionon endothelial cells through autocrine or paracrine action. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 2/ANGPTL2 Antibody (NBP1-79557)(1)

Publication using NBP1-79557

• Farhat Nada, Mamarbachi Aida M, Thorin Eric et al. Cloning, expression and purification of functionally active human angiopoietin-like protein 2. SpringerPlus 2014-01-01 [PMID: 25077060] (WB, Human)

Bioinformatics

• Gene Symbol: ANGPTL2
• Uniprot:
  • NP_036230()