Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate


Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate [NBL1-07524] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ANGPTL1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

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Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate Summary


Angiopoietin-like Protein 1/ANGPTL1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC208671

Accession#: NM_004673

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate

  • ANG-3
  • ANG3angioarrestin
  • Angiopoietin like Protein 1
  • angiopoietin Y1
  • angiopoietin-3
  • angiopoietin-like 1
  • Angiopoietin-like Protein 1
  • angiopoietin-related protein 1
  • ANGPT3
  • AngY
  • AngY1
  • ARP1
  • ARP1angiopoietin 3
  • dJ595C2.2 (angiopoietin Y1)
  • dJ595C2.2
  • KIAA0351
  • UNQ162


Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524) (0)

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Gene Symbol ANGPTL1