Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate

Images

 
Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate [NBL1-07524] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ANGPTL1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate Summary

Description
Transient overexpression lysate of angiopoietin-like 1 (ANGPTL1) The lysate was created in HEK293T cells, using Plasmid ID RC208671 and based on accession number NM_004673. The protein contains a C-MYC/DDK Tag.
Gene
ANGPTL1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate

  • ANG-3
  • ANG3angioarrestin
  • Angiopoietin like Protein 1
  • angiopoietin Y1
  • angiopoietin-3
  • angiopoietin-like 1
  • Angiopoietin-like Protein 1
  • angiopoietin-related protein 1
  • ANGPT3
  • ANGPTL1
  • AngY
  • AngY1
  • ARP1
  • ARP1angiopoietin 3
  • dJ595C2.2 (angiopoietin Y1)
  • dJ595C2.2
  • KIAA0351
  • UNQ162

Background

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

923-AN/CF
Species: Hu
Applications: BA
DANG20
Species: Hu
Applications: ELISA
PP-H7147-00
Species: Hu
Applications: IHC, IP, WB
PP-H8132-00
Species: Hu
Applications: IHC, IP, WB
AF964
Species: Hu
Applications: Block, IHC, WB
AF313
Species: Hu
Applications: Block, IHC, Simple Western, WB
AF3485
Species: Hu, Pm
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
AF1444
Species: Mu
Applications: IHC, WB
DAN00
Species: Hu
Applications: ELISA
AF5414
Species: Hu
Applications: Simple Western, WB
DVE00
Species: Hu
Applications: ELISA
NB500-207
Species: Hu
Applications: ICC/IF, IP, WB
AF2335
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, IP, WB
H00008518-M03
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-68205
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-30463
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IP, WB
H00010097-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
H00057180-B01P
Species: Hu
Applications: WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBL1-07524
Species: Hu
Applications: WB

Publications for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524) (0)

There are no publications for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524) (0)

There are no reviews for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Angiopoietin-like Protein 1/ANGPTL1 Products

Bioinformatics Tool for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524)

Discover related pathways, diseases and genes to Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524)

Discover more about diseases related to Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524).
 

Pathways for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524)

View related products by pathway.

PTMs for Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524)

Learn more about PTMs related to Angiopoietin-like Protein 1/ANGPTL1 Lysate (NBL1-07524).

Blogs on Angiopoietin-like Protein 1/ANGPTL1

There are no specific blogs for Angiopoietin-like Protein 1/ANGPTL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Angiopoietin-like Protein 1/ANGPTL1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ANGPTL1