Angiopoietin-4 Antibody (RM0073-2H34) [Janelia Fluor® 549]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0073-2H34
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 549

Angiopoietin-4 Antibody (RM0073-2H34) [Janelia Fluor® 549] Summary

• Immunogen: Mouse recombinant Angiopoietin-3
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: ANGPT4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Angiopoietin-4 Antibody (RM0073-2H34) [Janelia Fluor® 549]

• AGP4
• ANG3
• ANG-3
• ANG-4
• ANG4angiopoietin-3
• angiopoietin 4
• Angiopoietin-3
• Angiopoietin-4
• ANGPT4
• dJ824F16.2 (angiopoietin 4)
• MGC138181
• MGC138183

Background

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPT4