Angiopoietin-3 Antibody (113504) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB
• Clone: 113504
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Angiopoietin-3 Antibody (113504) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse Angiopoietin-3
• Specificity: Detects mouse Angiopoietin-3 in direct ELISA and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like factor, and rhAngiopoietin-4 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-3 Antibody (113504) [Alexa Fluor® 405]

• ANG3
• ANG4
• Angiopoietin-3
• ANGPT3

Background

Mouse Angiopoietin-3 (ANG-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse ANG-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46% and 54% aa sequence identity with mouse ANG-1, mouse ANG-2 and human ANG-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG-1 (97%) and ANG-2 (85%), mouse ANG-3 is believed to be an ortholog of human ANG-4 based on chromosomal localization studies (1, 2). Human ANG-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse ANG-3 has been reported to be a Tie-2 antagonist. It is likely that mouse ANG-3, like ANG-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.