Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. ...read more
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1 Ser20-Phe498 Accession # Q15389
Detects human Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2, recombinant mouse Angiopoietin-like 3, rhAngiopoietin-4, or rhAngiopoietin-like 7 is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Angiopoietin-1 Antibody (171718) [Unconjugated]
(Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted
ligands which bind with similar affinity to Tie-2, a receptor tyrosine
kinase with immunoglobulin and epidermal growth factor homology domains
expressed primarily on endothelial cells and early hematopoietic cells.
Tie-2 and angiopoietins have been shown to play critical roles in
embryogenic angiogenesis and in maintaining the integrity of the adult
vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa)
precursor protein that contains a coiled-coiled domain near the
amino-terminus and a fibrinogen-like domain at the C-terminus. Human
Ang-1 shares approximately 97% and 60% aa sequence identity with mouse
Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2
signaling on endothelial cells to promote chemotaxis, cell survival,
cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has
alternatively been reported to be an antagonist for Ang-1-induced Tie-2
signaling as well as an agonist for Tie-2 signaling, depending on the
cell context (5).
Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
Davis, S. et al. (1996) Cell 87:1161.
Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.