Angiopoietin-1 Antibody (171718) [Unconjugated]

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Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
171718
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

Angiopoietin-1 Antibody (171718) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
Ser20-Phe498
Accession # Q15389
Specificity
Detects human Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2, recombinant mouse Angiopoietin-like 3, rhAngiopoietin-4, or rhAngiopoietin-like 7 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ANGPT1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB923 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody (171718) [Unconjugated]

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa) precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% aa sequence identity with mouse Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1-induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

  1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  2. Davis, S. et al. (1996) Cell 87:1161.
  3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
  4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
  5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiopoietin-1 Antibody (MAB923)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 6 applications: ELISA Development, ICC, IHC, IHC-Fr, Immunoprecipitation, Western Blot.


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ELISA Development
(1)
ICC
(1)
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(1)
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Immunoprecipitation
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(2)
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Human
(3)
Mouse
(3)
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(1)
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Showing Publications 1 - 7 of 7.
Publications using MAB923 Applications Species
K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018;9(1):2448. 2018 [PMID: 29934496] (Western Blot, Mouse) Western Blot Mouse
Wilson Chan Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells PLoS ONE, 2016;11(6):e0156994. 2016 [PMID: 27304216] (ELISA Development, Human) ELISA Development Human
Khairoun M, van den Heuvel M, van den Berg B, Sorop O, de Boer R, van Ditzhuijzen N, Bajema I, Baelde H, Zandbergen M, Duncker D, Rabelink T, Reinders M, van der Giessen W, Rotmans J Early systemic microvascular damage in pigs with atherogenic diabetes mellitus coincides with renal angiopoietin dysbalance. PLoS ONE, 2015;10(4):e0121555. 2015 [PMID: 25909188] (IHC-Fr, Porcine) IHC-Fr Porcine
Cheng CL, Hou HA, Jhuang JY, Lin CW, Chen CY, Tang JL, Chou WC, Tseng MH, Yao M, Huang SY, Ko BS, Hsu SC, Wu SJ, Tsay W, Chen YC, Tien HF High bone marrow angiopoietin-1 expression is an independent poor prognostic factor for survival in patients with myelodysplastic syndromes. Br. J. Cancer, 2011;105(7):975-82. 2011 [PMID: 21878936] (IHC, Human) IHC Human
Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy. FASEB J., 2008;22(8):3010-23. 2008 [PMID: 18502941] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN Regulation of angiopoietin expression by bacterial lipopolysaccharide. Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L955-63. 2008 [PMID: 18310225] (Western Blot, Mouse) Western Blot Mouse
Hashiramoto A, Sakai C, Yoshida K, Tsumiyama K, Miura Y, Shiozawa K, Nose M, Komai K, Shiozawa S Angiopoietin 1 directly induces destruction of the rheumatoid joint by cooperative, but independent, signaling via ERK/MAPK and phosphatidylinositol 3-kinase/Akt. Arthritis Rheum., 2007;56(7):2170-9. 2007 [PMID: 17599743] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol ANGPT1
Uniprot