AMSH-LP Antibody

Western Blot: AMSH-LP Antibody [NBP2-20501] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: AMSH-LP Antibody [NBP2-20501] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STAMBPL1 protein stained by STAMBPL1 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: AMSH-LP Antibody [NBP2-20501] - CL1-5 xenograft, using STAMBPL1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

AMSH-LP Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AMSH-LP. The exact sequence is proprietary.
• Predicted Species: Mouse (94%), Rat (93%), Porcine (93%), Chimpanzee (99%), Bovine (93%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAMBPL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AMSH-LP Antibody

• ALMalpha
• ALMalpha;AMSH-FP;AMSH-like protease;AMSH-LP;bA399O19.2;STAMBPL1
• AMSH-FP
• AMSHLP
• AMSH-LP
• STAMBPL1

Background

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STAMBPL1
• Uniprot:
  • Q96FJ0()