AMPK alpha 1/2 Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line treated with 1 µM ionomycin for the indicated times in minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-AMPKa1/2 (T183/T172) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-AMPKa1/2 (T183/T172) at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Product Discontinued

AMPK alpha 1/2 Antibody Summary

• Immunogen: Phosphopeptide containing human AMPK alpha 1 T183 site
• Specificity: Detects human AMPK alpha 1 and AMPK alpha 2 when phosphorylated at T183 and T172, respectively, in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAA1
• Purity Statement: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK alpha 1/2 Antibody

• AMPK alpha 1/2
• AMPK
• AMPKa1
• PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPK alpha 1/2 Antibody (AF2509)(2)

Publications using AF2509

• Y Chen, F Qiu, B Yu, Y Chen, F Zuo, X Zhu, KS Nandakumar, C Xiao Metformin, an AMPK Activator, Inhibits Activation of FLSs but Promotes HAPLN1 Secretion Mol Ther Methods Clin Dev, 2020-05-13;17(0):1202-1214. 2020-05-13 [PMID: 32518807] (Western Blot, Human)
• Mir , Shakeel, Schwarze , Steven R, Jin , Ling, Zhang , Jinling, Friend , Woodrow, Miriyala , Sumitra, St Clair , Daret, Craven , Rolf J Progesterone receptor membrane component 1/Sigma-2 receptor associates with MAP1LC3B and promotes autophagy. Autophagy, 2013-09-04;9(10):1566-78. 2013-09-04 [PMID: 24113030] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRKAA1