Amphiphysin/AMPH Antibody

Images

 
Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - 50 ug rat brain lysate/extract 5% SDS-PAGE; antibody dilution 1:500.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.
Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - (30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000
Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - 50 ug mouse brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.
Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Order Details

Amphiphysin/AMPH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin. The exact sequence is proprietary.
Localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cytoplasm, cytoskeleton
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMPH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Amphiphysin/AMPH Antibody

  • AMPH
  • AMPH1
  • amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
  • amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • amphiphysin I
  • Amphiphysin

Background

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AMPH
Entrez