Aminopeptidase PILS/ARTS1 Antibody (S06-1H3)

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (S06-1H3) [NBP3-14916] - Western blot detection of Aminopeptidase PILS/ARTS1 in Hela, C6, Jurkat cell lysates using Aminopeptidase PILS/ARTS1Rabbit mAb (1:500 diluted). Predicted band size: 107kDa. Observed band size: 107kDa.

Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (S06-1H3) [NBP3-14916] - Immunohistochemistry of Aminopeptidase PILS/ARTS1 in paraffin-embedded Human lung cancer tissue using Aminopeptidase PILS/ARTS1 Rabbit mAb at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S06-1H3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Aminopeptidase PILS/ARTS1 Antibody (S06-1H3) Summary

• Description: Novus Biologicals Rabbit Aminopeptidase PILS/ARTS1 Antibody (S06-1H3) (NBP3-14916) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Aminopeptidase PILS/ARTS1 (Uniprot#: Q9NZ08)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERAP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20-1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/2000
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Aminopeptidase PILS/ARTS1 Antibody (S06-1H3)

• Adipocyte-derived leucine aminopeptidase
• A-LAP
• A-LAPALAP
• Aminopeptidase PILS
• ARTS1
• ARTS1aminopeptidase regulator of TNFR1 shedding
• ARTS-1PILSAP
• EC 3.4.11
• EC 3.4.11.-
• EC 3.4.11.1
• endoplasmic reticulum aminopeptidase 1
• endoplasmic reticulum aminopeptidase associated with antigen processing
• ERAAP1
• ERAP1
• KIAA0525APPILS
• PILS-AP
• PILS-APERAAP
• Puromycin-insensitive leucyl-specific aminopeptidase
• Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Background

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon-gamma. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERAP1