Aminopeptidase P1/XPNPEP1 Antibody

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - Analysis of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - Staining of Pig Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aminopeptidase P1/XPNPEP1 Antibody Summary

• Immunogen: Peptide with sequence C-LIRETQPISKQH corresponding to C-Terminus according to NP_001161076.1.
• Epitope: C-LIRETQPISKQH
• Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XPNPEP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in human heart, skeletal muscle and pancreas lysates and 75 kDa band observed in mouse and rat small intestines (calculated MW of 69.9 kDa band according to NP_065116.2).Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Pancreas.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aminopeptidase P1/XPNPEP1 Antibody

• Aminoacylproline aminopeptidase
• Aminopeptidase P1
• APP1
• Cytosolic aminopeptidase P
• cytosolic
• EC 3.4.11.9
• SAMP
• xaa-Pro aminopeptidase 1
• XPNPEP1
• XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like
• X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
• X-prolyl aminopeptidase 1, soluble

Background

X-prolyl aminopeptidase (EC 3.4.11.9) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. Because of its specificity toward proline, it has been suggested that X-prolyl aminopeptidase is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of X-prolyl aminopeptidase results in excretion of large amounts of imino-oligopeptides in urine (Blau et al., 1988 [PubMed 3141711]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase P1/XPNPEP1 Antibody (NBP1-51954)(1)

Publication using NBP1-51954

• Gevaert K, Goethals M, Martens L et al. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat Biotechnol 2003-05-01 [PMID: 12665801]

Bioinformatics

• Gene Symbol: XPNPEP1
• Entrez:
  • 7511(Human)
  • 170750(Mouse)
  • 170751(Rat)
• Uniprot:
  • NP_065116.2(Human)