Aminopeptidase P1/XPNPEP1 Antibody

Images

 
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - Analysis of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - Staining of Pig Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Aminopeptidase P1/XPNPEP1 Antibody Summary

Immunogen
Peptide with sequence C-LIRETQPISKQH corresponding to C-Terminus according to NP_001161076.1.
Epitope
C-LIRETQPISKQH
Specificity
This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XPNPEP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 80 kDa band observed in human heart, skeletal muscle and pancreas lysates and 75 kDa band observed in mouse and rat small intestines (calculated MW of 69.9 kDa band according to NP_065116.2).
Publications
Read Publication using NBP1-51954.

Reactivity Notes

Predicted cross-reactivity based on seqeunce identity: Bovine, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aminopeptidase P1/XPNPEP1 Antibody

  • Aminoacylproline aminopeptidase
  • Aminopeptidase P1
  • APP1
  • Cytosolic aminopeptidase P
  • cytosolic
  • EC 3.4.11.9
  • SAMP
  • xaa-Pro aminopeptidase 1
  • XPNPEP1
  • XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like
  • X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
  • X-prolyl aminopeptidase 1, soluble

Background

X-prolyl aminopeptidase (EC 3.4.11.9) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. Because of its specificity toward proline, it has been suggested that X-prolyl aminopeptidase is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of X-prolyl aminopeptidase results in excretion of large amounts of imino-oligopeptides in urine (Blau et al., 1988 [PubMed 3141711]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol XPNPEP1
Entrez
Uniprot