Aminopeptidase LRAP/ERAP2 Antibody

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Western Blot: Aminopeptidase LRAP/ERAP2 Antibody [NBP1-20913] - (0.5ug/ml) staining of Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Aminopeptidase LRAP/ERAP2 Antibody Summary

Immunogen
Peptide with sequence C-QYLKKFSYRNAKND corresponding to internal region according to NP_071745.1.
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ERAP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 110 kDa band observed in human spleen lysates (calculated MW of 110 kDa band according to NP_071745.1). An additional band of unknown identity was also consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-20913.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aminopeptidase LRAP/ERAP2 Antibody

  • Aminopeptidase LRAP
  • EC 3.4.11
  • EC 3.4.11.3
  • endoplasmic reticulum aminopeptidase 2
  • ERAP2
  • FLJ23633
  • FLJ23701
  • FLJ23807
  • Leukocyte-derived arginine aminopeptidase
  • LRAPL-RAPEC 3.4.11.-

Background

Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aminopeptidase LRAP/ERAP2 Antibody (NBP1-20913)(1)

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Bioinformatics

Gene Symbol ERAP2
Entrez
Uniprot