Aminopeptidase LRAP/ERAP2 Antibody

Western Blot: Aminopeptidase LRAP/ERAP2 Antibody [NBP1-20913] - (0.5ug/ml) staining of Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

Aminopeptidase LRAP/ERAP2 Antibody Summary

• Immunogen: Peptide with sequence C-QYLKKFSYRNAKND corresponding to internal region according to NP_071745.1.
• Epitope: C terminus
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human spleen lysates (calculated MW of 110 kDa band according to NP_071745.1). An additional band of unknown identity was also consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aminopeptidase LRAP/ERAP2 Antibody

• Aminopeptidase LRAP
• EC 3.4.11
• EC 3.4.11.3
• endoplasmic reticulum aminopeptidase 2
• ERAP2
• FLJ23633
• FLJ23701
• FLJ23807
• Leukocyte-derived arginine aminopeptidase
• LRAPL-RAPEC 3.4.11.-

Background

Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase LRAP/ERAP2 Antibody (NBP1-20913)(1)

Publication using NBP1-20913

• Saveanu L, Carroll O, Lindo V et al. Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum. Nat Immunol 2005-07-01 [PMID: 15908954]

Bioinformatics

• Gene Symbol: ERAP2
• Entrez:
  • 64167(Human)
• Uniprot:
  • NP_071745.1(Human)