Aminopeptidase A/ENPEP Antibody Summary
Immunogen |
Peptide with sequence C-EQYQKTSLAQEKEK corresponding to internal region according to NP_001968.3. |
Predicted Species |
Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
ENPEP |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 3 - 5 ug/mL
- Immunohistochemistry-Paraffin 3 - 5 ug/mL
- Peptide ELISA Detection limit 1:64000
- Western Blot 1 - 3 ug/mL
|
Application Notes |
WB: Approx 170 kDa band observed in human brain and testis lysates (calculated MW of 109 kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 11994381). IHC: Human kidney shows staining of basement membrane and the mesangium in the glomerulus. |
Control |
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Publications |
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Reactivity Notes
Rat reactivity in WB reported in scientific literature (PMID: 28399178).
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
Preservative |
0.02% Sodium Azide |
Concentration |
0.5 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for Aminopeptidase A/ENPEP Antibody
Background
Aminopeptidase A is a zinc-dependent metallopeptidase that selectively cleaves N-terminal Glu, or Asp residues from peptides. In mice, it is expressed on early B cells and on a subset of thymic cortical epithelial cells, but not on mature lymphocytes. Additionally, it is expressed on endothelial cells, intestinal epithelium, proximal renal tubules, and other non-hemopoietic tissues. In humans, angiotensin II is the the best-defined biologically active substrate of APA. Although its role in the immune response is still uncertain, its expression on renal cell carcinoma has been the focus of some cancer research studies.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Aminopeptidase A/ENPEP Antibody (NB100-1885)(1)
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Product General Protocols
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FAQs for Aminopeptidase A/ENPEP Antibody (NB100-1885) (0)
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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