Aminopeptidase A/ENPEP Antibody

Western Blot: Aminopeptidase A/ENPEP Antibody [NB100-1885] - Staining of human testis lysate (RIPA buffer, 30 ug total protein). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Aminopeptidase A/ENPEP Antibody [NB100-1885] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aminopeptidase A/ENPEP Antibody Summary

• Immunogen: Peptide with sequence C-EQYQKTSLAQEKEK corresponding to internal region according to NP_001968.3.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENPEP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx 170 kDa band observed in human brain and testis lysates (calculated MW of 109 kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 11994381). IHC: Human kidney shows staining of basement membrane and the mesangium in the glomerulus.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat reactivity in WB reported in scientific literature (PMID: 28399178).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aminopeptidase A/ENPEP Antibody

• Aminopeptidase A
• APA
• AP-A
• CD249 antigen
• CD249
• Differentiation antigen gp160
• EAP
• EC 3.4.11
• EC 3.4.11.7
• ENPEP
• glutamyl aminopeptidase (aminopeptidase A)
• glutamyl aminopeptidase
• gp160

Background

Aminopeptidase A is a zinc-dependent metallopeptidase that selectively cleaves N-terminal Glu, or Asp residues from peptides. In mice, it is expressed on early B cells and on a subset of thymic cortical epithelial cells, but not on mature lymphocytes. Additionally, it is expressed on endothelial cells, intestinal epithelium, proximal renal tubules, and other non-hemopoietic tissues. In humans, angiotensin II is the the best-defined biologically active substrate of APA. Although its role in the immune response is still uncertain, its expression on renal cell carcinoma has been the focus of some cancer research studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase A/ENPEP Antibody (NB100-1885)(1)

Publication using NB100-1885

• Ando H, Nagasaka T, Nomura M et al. Premenstrual disappearance of aminopeptidase A in endometrial stromal cells around endometrial spiral arteries/arterioles during the decidual change. J Clin Endocrinol Metab 2002-05-01 [PMID: 11994381]

Bioinformatics

• Gene Symbol: ENPEP
• Entrez:
  • 2028(Human)
  • 13809(Mouse)
• Uniprot:
  • Q07075(Human)