Aminoacylase/ACY1 Antibody (006) - Azide and BSA Free

Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Analysis of Human ACY1 expression on HepG2 cells. The cells were treated, stained with purified anti-Human ACY1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Staining of Human ACY1 in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89558 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 006
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Aminoacylase/ACY1 Antibody (006) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Aminoacylase/ACY1 (Accession#: NP_000657.1; Met1-Ser408).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACY1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Aminoacylase/ACY1 Antibody (006) - Azide and BSA Free

• ACY1
• ACY-1
• ACY1D
• acylase
• aminoacylase 1
• Aminoacylase
• aminoacylase-1
• EC 3.5.1
• EC 3.5.1.14
• N-acyl-L-amino-acid amidohydrolase

Background

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACY1