AMBRA1 Antibody

Images

 
Western Blot: AMBRA1 Antibody [NBP2-92849] - Analysis of extracts of various cell lines, using AMBRA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody [NBP2-92849] - Analysis of HeLa cells using AMBRA1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded rat lung using AMBRA1.
Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded mouse lung using AMBRA1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AMBRA1 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMBRA1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for AMBRA1 Antibody

  • activating molecule in BECN1-regulated autophagy protein 1
  • autophagy/beclin-1 regulator 1
  • DCAF3
  • DDB1 and CUL4 associated factor 3
  • FLJ20294
  • KIAA1736activating molecule in beclin-1-regulated autophagy
  • MGC33725
  • WD repeat domain 94
  • WDR94

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMBRA1 Antibody (NBP2-92849) (0)

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Secondary Antibodies

 

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Additional AMBRA1 Products

Array NBP2-92849

Bioinformatics Tool for AMBRA1 Antibody (NBP2-92849)

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Diseases for AMBRA1 Antibody (NBP2-92849)

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Blogs on AMBRA1.

Optogenetic Control of Mitophagy: AMBRA1 based mitophagy switch
By Christina Towers, PhD Mitophagy in the BrainSelective autophagic degradation of damaged mitochondria, known as mitophagy, has been described as a cyto-protective process. Accordingly, defects in mitophagy h...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

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Bioinformatics

Gene Symbol AMBRA1