AMBRA1 Antibody - BSA Free

Western Blot: AMBRA1 Antibody - BSA Free [NBP2-92849] - Western blot analysis of lysates from PANC-1 cells, using AMBRA1 Rabbit pAb at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded rat lung using AMBRA1 .

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded mouse lung using AMBRA1 .

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP2-92849] - Immunofluorescence analysis of NIH/3T3 cells using AMBRA1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AMBRA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AMBRA1 Antibody - BSA Free (NBP2-92849) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2). GAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMBRA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for AMBRA1 Antibody - BSA Free

• activating molecule in BECN1-regulated autophagy protein 1
• autophagy/beclin-1 regulator 1
• DCAF3
• DDB1 and CUL4 associated factor 3
• FLJ20294
• KIAA1736activating molecule in beclin-1-regulated autophagy
• MGC33725
• WD repeat domain 94
• WDR94

Background

AMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMBRA1