Home » alpha-Synuclein » alpha Synuclein Research Products » alpha-Synuclein [p Ser129] Antibody

alpha-Synuclein [p Ser129] Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - analysis of Human, Mouse brain lysate showing detection of approx. 16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: approx.16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - analysis of Human, Mouse brain lysate showing detection of approx. 16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 ...read more
Immunocytochemistry/ Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Primary hippocampal neurons treated with active ...read more
Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-61121AF405
Alexa Fluor 488
NBP2-61121AF488
Alexa Fluor 647
NBP2-61121AF647
Alexa Fluor 700
NBP2-61121AF700
Biotin
NBP2-61121B
DyLight 350
NBP2-61121UV
DyLight 405
NBP2-61121V
DyLight 488
NBP2-61121G
DyLight 550
NBP2-61121R
DyLight 650
NBP2-61121C
DyLight 680
NBP2-61121FR
DyLight 755
NBP2-61121IR
FITC
NBP2-61121F
HRP
NBP2-61121H
Janelia Fluor 549
NBP2-61121JF549
Janelia Fluor 646
NBP2-61121JF646
100% Guarantee on every product

alpha-Synuclein [p Ser129] Antibody Summary

Immunogen
Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Modification
p Ser129
Specificity
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNCA
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
A 1:1000 dilution of this Antibody was sufficient for detection of Alpha Synuclein pSer129 in 15 ug of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4 and 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Peptide affinity purified

Alternate Names for alpha-Synuclein [p Ser129] Antibody

  • alpha-Synuclein
  • I+/--synuclein
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • PARK1
  • PARK4
  • PD1
  • SNCA
  • synuclein alpha-140
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha
  • truncated alpha synuclein

Background

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Alpha synuclein phosphorylated at serine 129

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Tau [p Thr181]
Antibody
NB100-82245
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.
Immunohistochemistry-Paraffin: Tau [p Thr181] Antibody [NB100-82245] - analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr181) antibody.
Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Western blot analysis of extract from mouse brain tissue using Tau (Ab-181) antibody ( Lane 1 and 2) and Tau (phospho-Thr181) antibody ( Lane 3 and 4).
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, KD
LRRK2 Antibody
NB300-268
Western Blot: LRRK2 Antibody [NB300-268] - Western blotting of LRRK2. Recombinant LRRK2 (arrowhead) from transfected (+) HEK 293T and M17 cells was specifically recognized by all four LRRK2 antibodies (Ab1, Ab2, Ab3, and Ab4) used in this study. LRRK2 was not recognized in non-transfected cells (-). Image collected and cropped by CiteAb from the following publication (http://molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-1-17), licensed under a CC-BY licence.
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from verified customer review.
Knockout Validated: LRRK2 Antibody [NB300-268] - Mn increases LRRK2 kinase activity and expression in RAW 264.7 and HMC3 cells. Two-hundred (200) ug of protein were collected from cell lysates, followed by western blot analysis to determine the presence of LRRK2 in LRRK2 WT, KO RAW 264.7 and HMC3 cells. Beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0210248) licensed under a CC-BY licence.
(1 Review)
(40 Publications)
Species: Hu, Mu, Bv, Ce, I, Pl
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC, KO
beta-Synuclein
Antibody
NBP3-05529
Western Blot: beta-Synuclein Antibody [NBP3-05529] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a at a dilution of of 1:1,000 in green: [1] protein standard (red), [2] rat cortex [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 17 kDa corresponds to beta-Synuclein protein.
Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.
Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein Antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF, IHC
Park7/DJ-1 Antibody
NB300-270
Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Park7/DJ-1 Antibody [NB300-270] - Lysates of the human RPE cell lines ARPE-19 (lane 1) and D407 (lane 2), the mouse RPE cell line B6-RPE07 (lane 3), mouse primary RPE (lane 4) and mouse brain lysates (lane 5) were harvested and analyzed by immunoblot assay with DJ-1 antibody. The DJ-1 signal varied in intensity in each RPE cell culture. Each lane contained 20 ug of protein. Protein loads were confirmed in replicate blots probed with GAPDH. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB300-270] - Representative confocal micrographs of ARPE-19 monolayers plated on Transwells(R) and labeled with antibodies to DJ-1 (G,J) and COX IV (H,K). Under baseline conditions, there is very little colocalization between DJ-1 and COX IV, as observed in overlaid images (I); DJ-1 is mostly distributed through the cytoplasm (arrows). Upon oxidative stress induced by incubation with 400 uM H2O2 for 1 hr, DJ-1 staining is increased (arrowheads) in ARPE-19 (J) cultures. In cultures treated with H2O2 some DJ-1 re-distributed to mitochondria (arrowheads) and displayed significant colocalization with COX IV in overlaid images (L). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence.
(3 Reviews)
(19 Publications)
Species: Hu, Mu, Rt, Ch
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, In vitro
Tyrosine
Hydroxylase Antibody
NB300-109
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(7 Reviews)
(138 Publications)
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
beta Amyloid
Antibody (MOAB-2)
NBP2-13075
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).
(2 Reviews)
(31 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Synphilin-1
Antibody
NBP2-55955
Immunocytochemistry/Immunofluorescence: Synphilin-1 Antibody [NBP2-55955] - Staining of human cell line RT4 shows localization to cytoplasmic bodies.
Immunohistochemistry-Paraffin: Synphilin-1 Antibody [NBP2-55955] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PINK1 Antibody
BC100-494
Knockdown Validated: PINK1 Antibody [BC100-494] - HEK293 cells were co-transfected with PINK1 siRNA (#1 or #2) or scrambled siRNA (scrambled) and untagged wild-type (WT) or Ser65Ala (S65A) mutant Parkin as indicated using TransFectin reagent (Bio-Rad). 48 hrs post-transfection, cells were treated with/without 10 uM CCCP for 3 h. 0.25 mg of 1% Triton whole-cell lysate were subjected to immunoprecipitation with GST-Parkin antibody (S966C) covalently coupled to protein G Sepharose and then immunoblotted with anti-phospho-Ser65 antibody in the presence of dephosphorylated peptide. 5% of the IP was immunoblotted with total anti-Parkin antibody. 0.25 mg of whole-cell lysates were immunoprecipitated with anti-PINK1 antibody (S085D) and immunoblotted with anti-PINK1 antibody. Representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://rsob.royalsocietypublishing.org/cgi/doi/10.1098/rsob.120080) licensed under a CC-BY licence.
Western Blot: PINK1 Antibody [BC100-494] - Pathogenic mutants of Parkin are subjected to Ser65 phosphorylation. Phos-tag Western blotting for Parkin and Western blotting for PINK1 were performed using Parkin WT and a series of pathogenic mutants. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep01002) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/mL for 1h at room temperature, washed 3x in PBS and incubated with Alexa Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.
(12 Reviews)
(173 Publications)
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PEP-ELISA, PAGE, KD, KO
UCH-L1/PGP9.5
Antibody (31A3)
NB600-1160
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)
Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.
(1 Review)
(13 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ze
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
gamma-Synuclein
Antibody
NBP1-31209
Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - Synuclein-gamma Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Synuclein-gamma Antibody [NBP1-31209] - Paraffin-embedded mouse fore brain, gamma Synuclein antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Huntingtin
Antibody (JB89-34)
NBP2-75531
Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on A431 cell using anti-Huntingtin antibody at 1/500 dilution.
Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NLRP1/NALP1
Antibody
NBP1-54899
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.
Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] - Human Fetal Brain.
(9 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
Parkin Antibody
[Unconjugated]
AF1438
Western blot shows lysates of Sf 21 S. frugiperda insect ovarian cell line mock transfected or transfected GST tagged Parkin. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Parkin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1438) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GST tagged Parkin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Parkin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human Parkin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1438) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
Species: Hu
Applications: WB, IHC
Prion protein
Antibody (SC57-05)
NBP2-67569
Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of Prion Protein(PrP) on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Presenilin-1
Antibody (APS 18)
NB100-74510
Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.
Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 18) [NB100-74510] - Normal biopsies of deparaffinized Human liver tissue.
(1 Review)
(5 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Thyroid Peroxidase
Antibody
NBP1-97765
Western Blot: Thyroid Peroxidase Antibody [NBP1-97765] - (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-97765] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Chromogranin C
Antibody
NBP2-15895
Western Blot: Chromogranin C Antibody [NBP2-15895] - A. 50 ug mouse brain lysate/extract 7.5% SDS-PAGE Chromogranin C antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-15895] - Paraffin-embedded mouse fore brain. Chromogranin C antibody dilution: 1:500.
Western Blot: Chromogranin C Antibody [NBP2-15895] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Siglec-1/CD169
Antibody (HSn 7D2)
NB600-534
Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.
(1 Review)
(17 Publications)
Species: Hu, Mu, Po, V-Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA

Publications for alpha-Synuclein Antibody (NBP2-61121) (0)

There are no publications for alpha-Synuclein Antibody (NBP2-61121).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for alpha-Synuclein Antibody (NBP2-61121) (0)

There are no reviews for alpha-Synuclein Antibody (NBP2-61121). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Synuclein Antibody (NBP2-61121). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-61121AF405
Alexa Fluor 488 NBP2-61121AF488
Alexa Fluor 647 NBP2-61121AF647
Alexa Fluor 700 NBP2-61121AF700
Biotin NBP2-61121B
DyLight 350 NBP2-61121UV
DyLight 405 NBP2-61121V
DyLight 488 NBP2-61121G
DyLight 550 NBP2-61121R
DyLight 650 NBP2-61121C
DyLight 680 NBP2-61121FR
DyLight 755 NBP2-61121IR
FITC NBP2-61121F
HRP NBP2-61121H
Janelia Fluor 549 NBP2-61121JF549
Janelia Fluor 646 NBP2-61121JF646
Learn about Custom Antibody Labeling

Additional alpha-Synuclein Products

Bioinformatics Tool for alpha-Synuclein Antibody (NBP2-61121)

Discover related pathways, diseases and genes to alpha-Synuclein Antibody (NBP2-61121). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Synuclein Antibody (NBP2-61121)

Discover more about diseases related to alpha-Synuclein Antibody (NBP2-61121).
 

Pathways for alpha-Synuclein Antibody (NBP2-61121)

View related products by pathway.

PTMs for alpha-Synuclein Antibody (NBP2-61121)

Learn more about PTMs related to alpha-Synuclein Antibody (NBP2-61121).
 

Research Areas for alpha-Synuclein Antibody (NBP2-61121)

Find related products by research area.

Blogs on alpha-Synuclein.

Check out the latest blog posts on alpha-Synuclein.
Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.
Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.
The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our alpha-Synuclein [p Ser129] Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol SNCA