alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) - Azide and BSA Free

Analysis in human kidney and skeletal muscle tissues using NBP3-43876 antibody. Corresponding alpha-Methylacyl-CoA Racemase/AMACR RNA-seq data are presented for the same tissues.

Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.

Staining of human liver cancer shows strong granular cytoplasmic positivity in tumor cells.

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Analysis in human cell line CACO-2.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL9362
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q9UHK6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AMACR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9362) - Azide and BSA Free

• 2-methylacyl-CoA racemase
• alpha-Methylacyl-CoA Racemase
• alphaMethylacylCoARacemase
• AMACR
• AMACRD
• CBAS4
• EC 5.1.99.4
• RACE
• RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMACR