Alpha-mannosidase II Antibody

Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human parathyroid gland shows high expression.

Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining in human parathyroid gland and skeletal muscle tissues using anti-MAN2A1 antibody. Corresponding MAN2A1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha-mannosidase II Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKED
• Marker: Golgi Apparatus Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAN2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Alpha-mannosidase II Recombinant Protein Antigen (NBP1-89748PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Alpha-mannosidase II Antibody

• alpha-mannosidase 2
• AMan II
• EC 3.2.1
• EC 3.2.1.114
• Golgi alpha-mannosidase II
• golgi integral membrane protein 7
• GOLIM7
• Man II
• MANA2MANII
• Mannosidase alpha class 2A member 1
• mannosidase, alpha type II
• mannosidase, alpha, class 2A, member 1
• Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Background

Alpha-mannosidase II encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAN2A1