alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Al

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.

Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-500
• Theoretical MW: 66.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFP