Alpha Actinin (pan) Antibody

Product Discontinued

Alpha Actinin (pan) Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 30-60 of Human Actinin-alpha1.
• Marker: Cytoskeleton-Actin Filaments Marker
• Specificity: Alpha Actinin 1/2/3/4 antibody detects endogenous levels of total alpha-actinin protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  alpha-Actinin 1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Alpha Actinin (pan) Antibody

• actinin 1 smooth muscle
• actinin, alpha 1
• alpha-actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Focal adhesions were identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex, which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and alpha-actinin. In addition, vinculin, Talin and alpha-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. alpha-actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and talin complex or by a direct interaction with integrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1
• Entrez:
  • 87(Human)
  • 109711(Mouse)
  • 29437(Rat)
• Uniprot:
  • P12814()