Alkaline Phosphatase/ALPP/ALPI Antibody [DyLight 488]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: DyLight 488

Alkaline Phosphatase/ALPP/ALPI Antibody [DyLight 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Alkaline Phosphatase/ALPP
  Ile23-Asp506
  Accession # P05187
• Specificity: Detects recombinant human Alkaline Phosphatase Placental Type/ALPP and recombinant human Alkaline Phosphatase Intestinal Type/ALPI in direct ELISAs. Detects human ALPP and ALPI but not Alkaline Phosphatase Liver Type/ALPL in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ALPP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Alkaline Phosphatase/ALPP/ALPI Antibody [DyLight 488]

• Alkaline phosphatase Regan isozyme
• alkaline phosphatase, placental type
• Alkaline Phosphatase/ALPP/ALPI
• alkaline phosphomonoesterase
• ALP
• ALPI
• EC 3.1.3.1
• glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• PALP
• Placental alkaline phosphatase 1
• PLAP
• PLAP-1

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPP