ALK/CD246 Antibody (383625) [Allophycocyanin]

• Reactivity: Hu
• Applications: WB
• Clone: 383625
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

ALK/CD246 Antibody (383625) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALK/CD246
  Val19-Ser1038
  Accession # Q9UM73
• Specificity: Detects human ALK/CD246 in direct ELISAs and Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALK
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for ALK/CD246 Antibody (383625) [Allophycocyanin]

• ALK tyrosine kinase receptor
• ALK
• anaplastic lymphoma kinase (Ki-1)
• Anaplastic lymphoma kinase
• anaplastic lymphoma receptor tyrosine kinase
• CD246 antigen
• CD246
• EC 2.7.10.1
• Ki-1
• NBLST3
• Tcrz

Background

ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALK