Aldolase C Antibody (1A1) - BSA Free

Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.

Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (1A1) [NBP2-25144] - View of mixed neuron/glial cultures stained with NBP2-25144 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25144 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.

Flow Cytometry: Aldolase C Antibody (1A1) [NBP2-25144] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Aldolase C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: 1A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aldolase C Antibody (1A1) - BSA Free Summary

• Immunogen: The C-terminal 23 amino acids of human Aldolase C (KYEGSGEDGGAAAQSLYIANHAY). [UniProt# P09972]
• Marker: pan-Astrocyte Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDOC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Western Blot 1:2000-1:5000
• Application Notes: This Aldolase C (1A1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldolase C Antibody (1A1) - BSA Free

• ALDC
• aldolase 3
• aldolase C, fructose-bisphosphate
• Brain-type aldolase
• EC 4.1.2.13
• fructoaldolase C
• fructose-1,6-biphosphate triosephosphate lyase
• fructose-bisphosphate aldolase C

Background

Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene. Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, Aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with Aldolase A and 70% identity with Aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that Aldolase A and C are expressed in complementary cell types: Aldolase A mRNA is found in neurons; Aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cella. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDOC
• OMIM:
  • 103870(Human)