ALDH18A1 Antibody

Western Blot: ALDH18A1 Antibody [NBP1-52098] - (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ALDH18A1 Antibody Summary

• Immunogen: Peptide with sequence C-SEHGSLKYLH corresponding to C-Terminus according to NP_001017423.1.
• Epitope: C-SEHGSLKYLH
• Specificity: This antibody is expected to recognize both reported isoforms (NP_002851.2; NP_001017423.1).
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALDH18A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in lysates of NIH3T3 and MCF7 (calculated MW of 87.3 kDa band according to NP_002851.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH18A1 Antibody

• aldehyde dehydrogenase 18 family, member A1
• Aldehyde dehydrogenase family 18 member A1
• delta-1-pyrroline-5-carboxylate synthase
• EC 2.1.2.11
• EC 2.3.3.13
• GSASdelta1-pyrroline-5-carboxlate synthetase
• MGC117316
• P5CSdelta-1-pyrroline-5-carboxylate synthetase
• PYCSEC 1.2.1.41
• pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)

Background

This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH18A1 Antibody (NBP1-52098)(1)

Publication using NBP1-52098

• Rose J, Behm FM, Drgon T et al. Personalized smoking cessation: interactions between nicotine dose, dependence and quit success genotype score. Molecular medicine (Cambridge, Mass 2010-03-01 [PMID: 20379614]

Bioinformatics

• Gene Symbol: ALDH18A1
• Entrez:
  • 5832(Human)
  • 56454(Mouse)
  • 361755(Rat)
• Uniprot:
  • NP_002851.2(Human)