ALDH18A1 Antibody (NBP1-52098)

Images

 
Western Blot: ALDH18A1 Antibody [NBP1-52098] - (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ALDH18A1 Antibody Summary

Immunogen
Peptide with sequence C-SEHGSLKYLH corresponding to C-Terminus according to NP_001017423.1.
Epitope
C-SEHGSLKYLH
Specificity
This antibody is expected to recognize both reported isoforms (NP_002851.2; NP_001017423.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALDH18A1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 85 kDa band observed in lysates of NIH3T3 and MCF7 (calculated MW of 87.3 kDa band according to NP_002851.2).
Publications
Read Publication using NBP1-52098.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALDH18A1 Antibody

  • aldehyde dehydrogenase 18 family, member A1
  • Aldehyde dehydrogenase family 18 member A1
  • delta-1-pyrroline-5-carboxylate synthase
  • EC 2.1.2.11
  • EC 2.3.3.13
  • GSASdelta1-pyrroline-5-carboxlate synthetase
  • MGC117316
  • P5CSdelta-1-pyrroline-5-carboxylate synthetase
  • PYCSEC 1.2.1.41
  • pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)

Background

This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH18A1 Antibody (NBP1-52098)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALDH18A1
Entrez
Uniprot