Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Images

 
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - HEK293 overexpressing ALDH1A1 and probed with NB100-2563 (mock transfection in first lane).
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (1ug/ml) Analysis of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody Summary

Immunogen
Peptide with sequence C-RELGEYGFHEYTE corresponding to internal region according to NP_000680.2.
Marker
Astrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALDH1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 54.9 kDa band according to NP_000680.2).
IHC: Paraffin embedded Human Testis.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
Aldehyde Dehydrogenase 1-A1/ALDH1A1 Lysate (NBL1-07451)
Publications
Read Publications using NB100-2563.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

  • acetaldehyde dehydrogenase 1
  • ALDC
  • aldehyde dehydrogenase 1 family, member A1
  • aldehyde dehydrogenase 1, soluble
  • Aldehyde Dehydrogenase 1A1
  • Aldehyde Dehydrogenase 1-A1
  • Aldehyde dehydrogenase family 1 member A1
  • Aldehyde dehydrogenase, cytosolic
  • aldehyde dehydrogenase, liver cytosolic
  • ALDH class 1
  • ALDH1
  • ALDH11
  • ALDH1A1
  • ALDH-E1
  • ALHDII
  • EC 1.2.1
  • EC 1.2.1.36
  • MGC2318
  • PUMB1
  • RALDH 1
  • RALDH1
  • retinal dehydrogenase 1
  • retinaldehyde dehydrogenase 1

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (NB100-2563)(2)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALDH1A1
Entrez
Uniprot