Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (703410) [Unconjugated]

Aldehyde Dehydrogenase 1‑A1/ALDH1A1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Aldehyde Dehydrogenase 1‑A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 58 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Monoclonal Antibody (Catalog # MAB5869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, CyTOF-ready, ICC/IF
• Clone: 703410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (703410) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1-A1
  Ser2-Ser501
  Accession # P00352
• Specificity: Detects human Aldehyde Dehydrogenase 1-A1/ALDH1-A1 in direct ELISAs and Western blots. In direct ELISAs, approximatly
  50% cross‑reactivity with recombinant human Aldehyde Dehydrogenase 1-A2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (703410) [Unconjugated]

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Aldehyde dehydrogenases (ALDHs) are NAD(P)⁺-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

1. Marchitti, S.A. et al. (2008) Expert Opin. Drug Metab. Toxicol. 4:697.
2. Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.
3. King, G. and Holmes, R. (1997) Adv. Exp. Med. Biol. 414:19.
4. Jacobs, F.M. et al. (2007) Development 134:2673.
5. Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA. 103:11707.
6. Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869)(1)

Publication using MAB5869

• MV Barbolina Targeting Microtubule-Associated Protein Tau in Chemotherapy-Resistant Models of High-Grade Serous Ovarian Carcinoma Cancers, 2022-09-19;14(18):. 2022-09-19 [PMID: 36139693] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ALDH1A1
• Uniprot:
  • P00352()