Reactivity | HuSpecies Glossary |
Applications | WB, Simple Western, IHC, CyTOF-ready, ICC/IF |
Clone | 703410 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1-A1 Ser2-Ser501 Accession # P00352 |
Specificity | Detects human Aldehyde Dehydrogenase 1-A1/ALDH1-A1 in direct ELISAs and Western blots. In direct ELISAs, approximatly 50% cross‑reactivity with recombinant human Aldehyde Dehydrogenase 1-A2 is observed. |
Source | N/A |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | ALDH1A1 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | In Simple Western only 10-15 uL of the recommended dilution is used per data point. |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).
Publication using MAB5869 | Applications | Species |
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MV Barbolina Targeting Microtubule-Associated Protein Tau in Chemotherapy-Resistant Models of High-Grade Serous Ovarian Carcinoma Cancers, 2022-09-19;14(18):. 2022-09-19 [PMID: 36139693] (Western Blot, Human) | Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
Diseases for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869)Discover more about diseases related to Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869).
| Pathways for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869)View related products by pathway.
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PTMs for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869)Learn more about PTMs related to Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (MAB5869).
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