Albumin Antibody - BSA Free

Images

 
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from ...read more
Primary cell characterization.As per the materials and methods, tumors were isolated from mice and cultured. C) Table showing in vitro and in vivo growth characteristics of WT and APN KO primary cultures. Scale of ...read more
NLRP12 downregulates multiple JNK activating pathways in hepatocytes.(A) For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Albumin Antibody - BSA Free Summary

Immunogen
Full length protein (Mouse)
Specificity
By immunoelectrophoresis, the antiserum reacts specifically with mouse Albumin. No reactivity was detected against other mouse serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:30000
Application Notes
Use in IHC-Fr reported in scientific literature (PMID: 33277474). In ELISA, for coating plates use antibody at 1:100 - 1:500. Albumin antibody validated for WB from a verified customer review.
Control
Human Serum Albumin Overexpression Lysate
Reviewed Applications
Read 1 Review rated 2
using
NB600-41532 in the following applications:

Publications
Read Publications using
NB600-41532 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Albumin Antibody - BSA Free

  • ALB
  • Albumin
  • cell growth inhibiting protein 42
  • DKFZp779N1935
  • growth-inhibiting protein 20
  • PRO0883
  • PRO0903
  • PRO1341
  • serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (NB600-41532)(41)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB600-41532 Applications Species
Lama A, Pirozzi C, Severi I et al. Palmitoylethanolamide dampens neuroinflammation and anxiety-like behavior in obese mice Brain, behavior, and immunity 2022-02-14 [PMID: 35176443] (IF/IHC, Mouse) IF/IHC Mouse
O Shea TM, Wollenberg AL, Kim JH et al. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature communications 2020-12-04 [PMID: 33277474] (IHC-Fr, Mouse) IHC-Fr Mouse
Simoes S, Neufeld J. L, et al. Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. Sci Transl Med 2020-11-25 [PMID: 33239387] (WB, Mouse) WB Mouse
Ikeda A, Yamamoto T, Mineshiba J, Takashiba S Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells Sci Rep 2020-11-17 [PMID: 33203957] (ICC/IF, Mouse)

Details:
Immunocytochemistry performed on mouse salivary glands that were either positive or negative for CD49f.
ICC/IF Mouse
Yang AC, Stevens MY, Chen MB et al. Physiological blood-brain transport is impaired with age by a shift in transcytosis Nature 2020-07-01 [PMID: 32612231] (IF/IHC, Mouse) IF/IHC Mouse
Udden, S N, Kwak, Y T Et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife 2019-04-16 [PMID: 30990169] (WB, Human) WB Human
Walker S, Wankell M, Ho V et al. Targeting mTOR and Src restricts hepatocellular carcinoma growth in a novel murine liver cancer model PLoS ONE 2019-02-22 [PMID: 30794695] (WB, Mouse) WB Mouse
knittelfelder O, Traikov S, VvedenskaYa O et al. Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues. Anal. Chem. 2018-07-30 [PMID: 30004672] (WB, Mouse) WB Mouse
Janardhan KS, Jensen H, Clayton NP, Herbert RA Immunohistochemistry in Investigative and Toxicologic Pathology Toxicol Pathol. 2018-07-01 [PMID: 29966501] (IF/IHC, Mouse)

Details:
Citation used the HRP format of this antibody.
IF/IHC Mouse
Stradiot L, Verhulst S, Roosens T et al. Functionality based method for simultaneous isolation of rodent hepatic sinusoidal cells. Biomaterials. 2017-09-01 [PMID: 28595132]
Show All 41 Publications.

Review for Albumin Antibody (NB600-41532) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-41532:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  2
reviewed by:
Verified Customer
IHC-Fr Mouse 08/05/2022
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotA18

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Albumin Antibody (NB600-41532) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Albumin Products

Research Areas for Albumin Antibody (NB600-41532)

Find related products by research area.

Blogs on Albumin.

Albumin - a family of globular protein
Albumin is a soluble and globular monomeric protein encoded by chromosome 4 that comprises about half of the protein found in blood serum. It functions as a carrier protein for steroids, fatty acids, and thyroid hormones as well as stabilizing extrace...  Read full blog post.

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Recent Reviews

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Verified Customer
08/05/2022
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol ALB
Entrez
Uniprot