Albumin Antibody [HRP]

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Western Blot: Albumin Antibody [HRP] [NB110-16329] - WB analysis of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Albumin Antibody [HRP] Summary

Immunogen
Full length protein (Mouse).
Specificity
By immunoelectrophoresis, the antiserum reacts specifically with mouse Albumin. No reactivity was detected against other mouse serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:30000
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:100-1:250
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB110-16329 in the following applications:

Publications
Read Publication using
NB110-16329 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 0.2% BSA
Preservative
0.1% Pro-Clean 400
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Albumin Antibody [HRP]

  • ALB
  • Albumin
  • cell growth inhibiting protein 42
  • DKFZp779N1935
  • growth-inhibiting protein 20
  • PRO0883
  • PRO0903
  • PRO1341
  • serum albumin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Albumin Antibody (NB110-16329)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


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Review for Albumin Antibody (NB110-16329) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB110-16329:
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WB
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Images Ratings Applications Species Date Details
Western Blot Albumin NB110-16329
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reviewed by:
DIONYSIOS CHARTOUMPEKIS
WB Mouse 09/11/2012
View

Summary

ApplicationWestern Blot
Sample TestedMouse Liver Ripa whole extracts
SpeciesMouse
Lotp-11
CommentsThis antibody actually worked without any problems using the aforementioned conditions. As albumin is highly expressed in the liver I had to use 25 ug of protein extracts instead of 50ug that I usually use so as to avoid signal saturation. The 1:8000 dilution of the primary antibody seems appropriate for the western blot purposes. It is also noteworthy that after the incubation with the primary antibody as well as after the incubation with the secondary antibody I performed 3 washing steps (5 min each) using mQH2O,TBS-Tween1x, TBS-Tween 1x in each step correspondingly.

Blocking

Blocking Details5% skim milk in TBS-Tween 1x O/N at 4C

Primary Anitbody

Dilution Ratio1:8000, 3hours, RT in 5% skim milk in TBS-Tween 1x

Secondary Antibody

Secondary DescriptionRabbit anti-goat IgG (H+L),HRP
Secondary Manufacturer Cat#81-1620 Invitrogen
Secondary Concentration1:4000 dilution in 5% skim milk in TBS-Tween 1x

Details

Detection NotesChemiluminescence, Perkin Elmer, Western Lightning. Exposure 150sec. Detection using Biorad Chemidoc XRS+

Comments

CommentsThis antibody actually worked without any problems using the aforementioned conditions. As albumin is highly expressed in the liver I had to use 25 ug of protein extracts instead of 50ug that I usually use so as to avoid signal saturation. The 1:8000 dilution of the primary antibody seems appropriate for the western blot purposes. It is also noteworthy that after the incubation with the primary antibody as well as after the incubation with the secondary antibody I performed 3 washing steps (5 min each) using mQH2O,TBS-Tween1x, TBS-Tween 1x in each step correspondingly.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Albumin Antibody (NB110-16329) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Albumin Antibody (NB110-16329)

Discover related pathways, diseases and genes to Albumin Antibody (NB110-16329). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Albumin Antibody (NB110-16329)

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Blogs on Albumin.

Albumin - a family of globular protein
Albumin is a soluble and globular monomeric protein encoded by chromosome 4 that comprises about half of the protein found in blood serum. It functions as a carrier protein for steroids, fatty acids, and thyroid hormones as well as stabilizing extrace...  Read full blog post.

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DIONYSIOS CHARTOUMPEKIS
09/11/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ALB
Entrez