Albumin Antibody (188835) [Alexa Fluor® 488] - Serum

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1455G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 188835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Albumin Antibody (188835) [Alexa Fluor® 488] - Serum Summary

• Immunogen: Human Serum Albumin
• Specificity: Detects human Serum Albumin in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Albumin Antibody (188835) [Alexa Fluor® 488] - Serum

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (IC1455G)(2)

Publications using IC1455G

• J Zhou, Z Wu, J Hu, D Yang, X Chen, Q Wang, J Liu, M Dou, W Peng, Y Wu, W Wang, C Xie, M Wang, Y Song, H Zeng, C Bai High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations Sci Adv, 2020-11-20;6(47):. 11/20/2020 [PMID: 33219024]
• M Pyzik, T Rath, TT Kuo, S Win, K Baker, JJ Hubbard, R Grenha, A Gandhi, TD Krämer, AR Mezo, ZS Taylor, K McDonnell, V Nienaber, JT Andersen, A Mizoguchi, L Blumberg, S Purohit, SD Jones, G Christians, WI Lencer, I Sandlie, N Kaplowitz, DC Roopenian, RS Blumberg Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury Proc. Natl. Acad. Sci. U.S.A, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330995] (Flow Cytometry, Canine)

Bioinformatics

• Gene Symbol: ALB