Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.
Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415] - Staining of human skin at 5 ug/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed ...read more
Simple Western: Alas1 Antibody [NB100-56415] - Simple Western lane view shows a specific band for Alas1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human, chimp and beluga whale; 94% in dog and chicken; 88% in mouse, rat and lungfish; 83% in bovine, xenopus, pufferfish; 77% in coelacanth. Rat reactivity reported in scientific literature (PMID: 19467246).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 188.8.131.52) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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