Alas1 Antibody

Images

 
Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.
Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415] - Staining at 5 ug/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed by alkaline ...read more
Simple Western: Alas1 Antibody [NB100-56415] - Simple Western lane view shows a specific band for Alas1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Rt, Ca, Ch, PmSpecies Glossary
Applications WB, Simple Western, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Alas1 Antibody Summary

Immunogen
A portion of amino acids 480-530 of human ALAS-1 were used as the immunogen.
Predicted Species
Chicken (94%), Canine (94%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALAS1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control
Alas1 Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-56415 in the following applications:

  • WB
    3 publications

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human, chimp and beluga whale; 94% in dog and chicken; 88% in mouse, rat and lungfish; 83% in bovine, xenopus, pufferfish; 77% in coelacanth. Rat reactivity reported in scientific literature (PMID: 19467246).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Alas1 Antibody

  • 5-aminolevulinic acid synthase 1
  • ALAS
  • ALAS3
  • ALASH
  • ALAS-H
  • aminolevulinate, delta-, synthase 1
  • Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial
  • Delta-aminolevulinate synthase 1
  • EC 2.3.1
  • EC 2.3.1.37
  • MIG4
  • migration-inducing protein 4

Background

Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alas1 Antibody (NB100-56415)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALAS1
Entrez
Uniprot