AKT1 [p Thr308] Antibody

Images

 
Western Blot: Akt1 [p Thr308] Antibody [NSB602] - Peptide Competition and Phosphatase Stripping: Extracts prepared from HEK293 cells co-expressing RasV12 and RacL61 were resolved by SDS-PAGE on a 10% polyacrylamide gel ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AKT1 [p Thr308] Antibody Summary

Immunogen
Synthetic peptide derived from a region of AKT that contains threonine 308.
Modification
p Thr308
Localization
Cytoplasmic and Nuclear
Specificity
AKT [Thr308]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
WB: Use at a concentration of 1:1000. Predicted molecular weight: 65 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKT1 [p Thr308] Antibody

  • AKT
  • Akt1
  • EC 2.7.11
  • EC 2.7.11.1
  • PKB alpha
  • PKBMGC99656
  • PRKBA
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1

Background

Phosphorylation site specific antibody selective for the phosphorylated form of the AKT protein containing a phosphate on threonine 308. The antibody has been shown to recognize AKT in NIH-3T3 cells stimulated with PDGF. AKT is a key effector of the PI3K pathway. Several studies have shown that a signalling pathway from PI3K to AKT may mediate some cellular responses of PI3K, including protection from apoptosis in PC12 cells. AKT accomplishes its cell survival-promoting function by inhibiting apoptosis by means of its ability to phosphorylate and inactivate BAD and caspase 9. The function of AKT is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (threonine 308) in the kinase core and hydrophobic phosphorylation site on the carboxyl terminus (serine 473). Threonine 308 is phosphorylated by the phosphoinositide-dependent kinase 1 (PDK 1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46403
Species: Hu
Applications: IHC
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF847
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF3978
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for AKT1 Antibody (NSB602) (0)

There are no publications for AKT1 Antibody (NSB602).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AKT1 Antibody (NSB602) (0)

There are no reviews for AKT1 Antibody (NSB602). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AKT1 Antibody (NSB602). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Secondary Antibodies

 

Isotype Controls

Additional AKT1 Products

Bioinformatics Tool for AKT1 Antibody (NSB602)

Discover related pathways, diseases and genes to AKT1 Antibody (NSB602). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AKT1 Antibody (NSB602)

Discover more about diseases related to AKT1 Antibody (NSB602).
 

Pathways for AKT1 Antibody (NSB602)

View related products by pathway.

PTMs for AKT1 Antibody (NSB602)

Learn more about PTMs related to AKT1 Antibody (NSB602).
 

Research Areas for AKT1 Antibody (NSB602)

Find related products by research area.

Blogs on AKT1. Showing 1-10 of 11 blog posts - Show all blog posts.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Antibodies Targeting the mTOR Pathway for Many Diseases
The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AKT1 [p Thr308] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol AKT1
Entrez