AKT1 Antibody Pair

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal

Order Details

AKT1 Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: mouse monoclonal anti-AKT1 (300 ug)
  • Antibody pair for WB: rabbit polyclonal anti-AKT1 (50 ul)
  • Clonality
    Polyclonal
    Gene
    AKT1
    Purity
    Immunogen affinity purified

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for AKT1 Antibody Pair

    • AKT
    • Akt1
    • EC 2.7.11
    • EC 2.7.11.1
    • PKB alpha
    • PKBMGC99656
    • PRKBA
    • Protein kinase B
    • Proto-oncogene c-Akt
    • rac protein kinase alpha
    • RAC-alpha serine/threonine-protein kinase
    • RAC-alpha
    • RAC-PK-alpha
    • RACPKB-ALPHA
    • v-akt murine thymoma viral oncogene homolog 1

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for AKT1 Antibody Pair (H00000207-PW1). (Showing 1 - 1 of 1 FAQ).

    1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
      • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

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    Blogs on AKT1. Showing 1-10 of 11 blog posts - Show all blog posts.

    Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
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    Altered expression of BCL2 in cancer
    Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

    AKT1 - Regulating cell growth and survival through phosphorylation
    AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

    Akt1 - a central player in cell survival signaling
    Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

    AKT1, Scene 1: The Cell Must Go On
    Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

    Caspase 9 and Mitochondrial Apoptosis Regulation
    Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

    Mapping Signal Transduction with mTOR Antibodies
    The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

    EZH2: Epigenetic Regulation Made Easy!
    Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

    Antibodies Targeting the mTOR Pathway for Many Diseases
    The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

    AKT Antibody Assays: A Complex Area with an Easy Solution
    We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

    Showing 1-10 of 11 blog posts - Show all blog posts.

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    Bioinformatics

    Gene Symbol AKT1
    Entrez